Assembly kinetics and identification of precursor proteins of complex I from Neurospora crassa

Complex I from the fungus Neurospora crassa.

Respiratory chain complex I is a complicated enzyme of mitochondria, that couples electron transfer from NADH to ubiquinone to the proton translocation across the inner membrane of the organelle. The fungus Neurospora crassa has been used as one of the main model organisms to study this enzyme. Complex I is composed of multiple polypeptide subunits of dual genetic origin and contains several pr...

Identification of the TYKY homologous subunit of complex I from Neurospora crassa.

A polypeptide subunit of complex I from Neurospora crassa, homologous to bovine TYKY, was expressed in Escherichia coli, purified and used for the production of rabbit antiserum. The mature mitochondrial protein displays a molecular mass of 21280 Da and results from cleavage of a presequence consisting of the first 34 N-terminal amino acids of the precursor. This protein was found closely assoc...

functional study of p0 proteins of two cereal yellow dwarf viruses (cydv-rpv and cydv-rps) and identification of their cellular partner

نقش سرکوبگری پروتئین p0 در دو پولروویروس کوتولگی زردی غلات cydv-rpv) و (cydv-rps، متفاوت در شدت بیماریزایی، مورد مطالعه قرار گرفت. نتایج نشان داد که هر دو پروتئین p0 p0cy-rpv) و (p0cy-rps قادر به سرکوب خاموشی آر ان ای ایجاد شده توسط ترادف های تراژن سنس و تکرار معکوس در n. benthamiana هستند. نشان داده شد که هر دو پروتئین p0 می توانند تخریب پروتئین argonaute-1 را تسهیل کنند. علاوه بر این، تمایل م...

Tryptophan transport in Neurospora crassa. I. Specificity and kinetics.

Wiley, W. R. (Battelle Memorial Institute, Richland, Wash.), and W. H. Matchett. Tryptophan transport in Neurospora crassa. I. Specificity and kinetics. J. Bacteriol. 92:1698-1705. 1966.-The transport of tryptophan in Neurospora crassa is mediated by a distinct stereospecific system which is chemically specific for a family of neutral amino acids. The process shows typical saturation kinetics a...

Mitochondrial ATPase complex from Neurospora crassa.